Impact of media components from different suppliers on enterokinase productivity in Pichia pastoris
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acknowledge the importance of e-commerce to their countries and to survival of their businesses and in creating and encouraging an atmosphere for the wide adoption and success of e-commerce in the long term. the investment for implementing e-commerce in the public sector is one of the areas which is focused in government‘s action plan for cross-disciplinary it development and e-readiness in go...
Cultivation strategies to enhance productivity of Pichia pastoris: A review.
Pichia pastoris, a methylotrophic yeast, is an established system for the production of heterologous proteins, particularly biopharmaceuticals and industrial enzymes. To maximise and optimise the production of recombinant products, recent molecular research has focused on numerous issues including the design of expression vectors, optimisation of gene copy number, co-expression of secretory pro...
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A recombinant Pichia pastoris containing enterokinase light chain gene from bovine was cultured in flasks firstly at different pH, methanol addition, and cell mass concentration for enterokinase production. Activity of enterokinase increased as cell mass concentration increased at more than = 6 % methanol added. No significant change of activity of enterokinase was observed when methanol added ...
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Leishmaniasis is a major infectious disease of considerable public health in more than 86 countries around the world. Several approaches toward vaccine development against this disease have been taken. Glycoprotein (gp63) is conserved among diverse species of Leishmania and has induced immunological responses in murine models. Therefore, this glycoprotein has been considered as a second generat...
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Recombinant protein production in microorganisms is one of the most studied areas of research in biotechnology today. In this respect the yeast Pichia pastoris is an important microbial production host due to its capability of secreting the target protein and performing posttranslational modifications. In a recent study, we described the development of a robust bioprocess for a glyco-engineered...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2021
ISSN: 1472-6750
DOI: 10.1186/s12896-021-00681-y